12:21pm UK, Saturday December 24, 2011
The child is the second for the couple, who already have a son, Elliot, born in 1998.
De Niro, 68, and his 56-year-old wife have called the newborn Helen Grace Hightower De Niro.
She weighed a healthy 7 lbs 2 oz at birth, the spokesman revealed.
The double-Oscar winner and star of Goodfellas, Raging Bull and Meet The Parents married his actress wife in 1997.
He also has a son and adopted daughter with ex-wife Diahnne Abbott and two twin sons with former girlfriend Toukie Smith.
The actor can currently be seen in the star-studded, feel-good film New Year's Eve.